pOR1GFP + scCro DNA pOR1scCroGFP.fasta.New plasmid constructed by Yin Gu from pUA139 and Hybrid P R-lacO promoter oligonucleotides pOR1GFP.fasta.Hybrid P R-lacO promoter oligonucleotides PR-lacO_oligos.txt.pUA元06 (guesstimate PDF summary pUALuxBX.pdf | guesstimate fasta sequence pUALuxBX.fasta ) BamHI and XhoI sites upstream of has luxCDABE kan R and pSC101 origin.pUA139 is similar to pMS201 (Map and sites pMS201.html | genbank sequence pMS201.genbank) except BamHI and XhoI sites upstream of GFPmut2 are reversed (Bam-Xho-GFP), kan R and pSC101 origin.pAH125 (PDF summary pAH125.pdf | genbank sequence pAH125.genbank | ncbi link) is a promoterless lacZ reporter plasmid, with kan R, OriR and Att lambda for generation of single copy integrants.Here is an annotated plain text sequence file of the important parts. pUCOR_Cro_lac same as pucOR_Cro but with translation start of lac Zalpha reconstructed.pucOR_Cro lambda OR-Olac control region replaces the Tac promoter of puCroRS.pET33bscCro8.fasta Reverse complement of pET33b sequence above with scCro 8 sequence cloned between NdeI and XhoI sites.pscCro16 single chain dimer, 16 residue linker in pET21a (reverse complement).pscCro8 single chain dimer, 8 residue linker in pET21a (reverse complement) Interactive HTML from sequence extractor.pJF1a Cro in pET21a with C-terminal His 6 fusion (91 Amino Acids).Cro K56 F58W fragment (EcoRI) - Tac promoter and synthetic Cro monomer gene.pUCromDG.fasta like pUCroRs but contains a small insertion in the Cro gene.pUC119CroRX like pUC118CroRX but EcoR1 to hindIII segment is reversed.pUC118CroRX like pUCroRS but includes terminator from pAP120.Cro substitution mutant - tryptophan for phenylalanine at 58 Reverse Complement pUCroF58W_RSrc.fasta.Interactive HTML from sequence extractor. pUCroRS.fasta and Reverse Complement ( pUCroRSrc.fasta) EcoRI - Tac promoter and synthetic Cro gene - (SmaI) of pAP120 inserted in pUC18.Here is a graphical genealogy Cro Plasmid Maps (PDF) of Cro expression plasmids from the Sauer Lab.pUC118.fasta Official pUC118 genbank entry at NCBI.pUC18.fasta Here is a link to the official pUC18 genbank entry at NCBI.
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